Flag: 5-10mg*10 vials

Product Description: Flag (Peptide)

Introduction:

Flag is a peptide commonly used in research settings, particularly in studies involving protein expression and purification. It is often utilized as a tag in molecular biology experiments to facilitate the identification and purification of proteins of interest. Flag, like other peptide tags, plays a crucial role in enhancing the detection, isolation, and analysis of specific proteins. Researchers around the world incorporate Flag into their experiments for a range of applications, including the study of protein interactions, structural analysis, and biochemical pathways.

What is Flag?

Flag is a short peptide sequence, typically consisting of a series of amino acids, that is used in molecular biology as a fusion tag for protein identification. The Flag peptide is frequently incorporated into recombinant proteins to make it easier for scientists to detect, purify, and study those proteins. This peptide is commonly used in protein research due to its ease of incorporation into proteins and its ability to facilitate various laboratory techniques such as affinity chromatography, Western blotting, and immunoprecipitation.

Though Flag itself is not a naturally occurring peptide in organisms, its synthetic design makes it an invaluable tool for biotechnological and biochemical research. The peptide can be added to the N- or C-terminus of recombinant proteins, allowing researchers to easily track and isolate the proteins during experimentation.

Potential Different Names:

• Flag Tag
• Flag Peptide Tag
• FLAG® Tag (trademarked by Sigma-Aldrich)
• Flag Fusion Protein Tag

Chemical Formula:

• Chemical Formula: C38H58N6O10

Structure:

The Flag peptide sequence is typically composed of eight amino acids: DYKDDDDK. These amino acids form a distinctive tag sequence that can be easily recognized by specific antibodies. The structure of Flag is simple, consisting of a short, linear arrangement of amino acids, with the specific sequence making it highly recognizable by certain antibodies during immunodetection techniques.

In many applications, Flag is incorporated into recombinant proteins, and its presence in the protein's structure allows for straightforward identification and isolation through specific binding interactions with anti-Flag antibodies. This makes it a highly versatile tool for protein research and purification.

How Does It Work?

Flag works primarily as a molecular tag used for the detection and purification of recombinant proteins. When researchers incorporate Flag into a recombinant protein, they can subsequently use anti-Flag antibodies to identify and isolate that protein from a mixture of cellular components.

Flag peptides are used in various laboratory techniques, including:

• Affinity chromatography: Anti-Flag antibodies can be attached to a column, and when a protein containing the Flag tag is introduced, it will bind to the antibodies, allowing for purification of the protein.
• Western blotting: Flag-tagged proteins can be detected using anti-Flag antibodies in Western blotting procedures.
• Immunoprecipitation: Proteins with the Flag tag can be precipitated out of solution using Flag-specific antibodies, enabling detailed analysis of protein interactions and complexes.

The functionality of Flag is mainly dependent on its ability to be recognized by the corresponding antibody, making it a useful tool for biochemists and molecular biologists who require efficient ways to study and purify proteins.

Conclusion:

Flag is a synthetic peptide tag widely used in scientific research for protein detection and purification. Its simple structure and ability to bind to specific antibodies make it an essential tool in various molecular biology techniques. As a peptide tag, it serves to facilitate the study and analysis of recombinant proteins, playing an important role in experiments involving protein identification, isolation, and analysis. The incorporation of Flag into proteins offers researchers an easy and efficient way to track and study protein function and interaction in the laboratory.

Disclaimer:

Flag is intended for research purposes only. It is not suitable for human consumption, and any use outside of professional research settings should be strictly avoided. The product should be handled only by qualified professionals in regulated laboratory environments. All users must comply with applicable safety guidelines, legal requirements, and ethical standards in their research practices.